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本研究选用新生 Wistar雄性大鼠 ,分为对照组和实验组。实验组于出生后 2— 10 d隔日皮下注射10 %谷氨酸单钠 (monosodium glutamate- MSG) (4 m g/ g体重 ) ,对照组在相应时间注射等量生理盐水 ,分别于出生后不同发育时期对眼球作肉眼及光镜观察 ,并作图像分析处理。结果显示 ,发育不同时期实验组眼球重量明显低于对照组 ;实验组视网膜的节细胞层 ,内核层 ,内网层及外网层厚度与对照组相比明显变薄 ;节细胞 ,双极细胞表现出早期水肿和迟发性固缩死亡 ,细胞数量减少。同时也观察到节细胞 ,双极细胞受 MSG损伤的时相及程度不等 ,从新生期至成年期呈进行性加重。视细胞变化不明显。本文结果表明谷氨酸单钠对视网膜神经细胞有明显的毒性作用 ,从而造成视觉功能障碍。
In this study, newborn Wistar male rats were divided into control group and experimental group. In the experimental group, 10% monosodium glutamate-MSG (4 mg / g body weight) was injected subcutaneously every other day after birth and the control group was injected with the same amount of saline at the corresponding time, Period of the eye for the naked eye and light microscopy, and for image analysis and processing. The results showed that the ocular weight of the experimental group was significantly lower than that of the control group in different developmental stages. The thickness of the retinal ganglion cell layer, the inner nuclear layer, the inner and outer retinal layers in the experimental group were significantly thinner than those in the control group. The number of ganglion cells, bipolar cells Early edema and delayed systolic death showed that the number of cells decreased. At the same time, it was also observed that the number of ganglion cells and bipolar cells varied with the time and extent of MSG injury, and progressed from neonatal to adulthood. Depending on cell changes is not obvious. Our results show that monosodium glutamate has obvious toxic effects on retinal nerve cells, resulting in visual dysfunction.