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本文系统地比较了国际上常用蛋白提取剂对小麦品种蛋白图谱的影响.在过夜静置提取下,15%、20%和25%2-氯乙醇提取的蛋白图谱相同,三者的抗对流性均较强.以电泳图谱上总谱带数而论,2-氯乙醇、乙二醇和70%乙醇的表现相同.但若以较清晰的谱带数而言,2-氯乙醇优于其余两种提取剂.乙二醇提取效率和蛋白图谱着色力较差,但其谱带分辩率却最好.70%乙醇提取离心后,必须加入蔗糖,以增强抗对流和蛋白谱带的染色效果.以1mol/L尿素作提取剂,在电泳图谱上会有新谱带出现或改变谱带的染色深浅,但图谱底色深、谱带易褪色.乙二醇提取的比25%2-氯乙醇的谱带迁移慢0.5～1mm.1%巯基乙醇能大量增加高分子量ω区的谱带,同时使其它区出现新谱带.ZY型提取剂比国际常用提取剂可使小麦品种蛋白图谱更丰富,其提取时间应不短于4h.
This article systematically compared the international commonly used protein extract on the wheat protein profile.After overnight static extraction, 15%, 20% and 25% 2-chloroethanol extract the same protein profiles, the three anti-convection 2-chloroethanol, ethylene glycol and 70% ethanol showed the same performance with respect to the number of bands in the electropherogram, but if the number of bands was clearer, 2-chloroethanol was superior to the other two The extraction efficiency of ethylene glycol and the poor coloring ability of the protein profile, but the best resolution of the band, 70% ethanol extraction after centrifugation, sucrose must be added to enhance the anti-convection and protein bands staining. With 1mol / L urea as extractant, there will be a new band on the electrophoresis spectrum to change the color of the band, but the background of the spectrum is dark and the band is easy to fade.Ethylene glycol is better than 25% 2-chloroethanol Of the band migration slow 0.5 ~ 1mm.1% Mercaptoethanol can significantly increase the high molecular weight ω region of the band, while the other regions appear new bands.ZY-type extractant than the international commonly used extractants can make the wheat variety protein map more abundant , The extraction time should not be shorter than 4h.