以高感玉米茎腐病自交系掖478为材料,用带禾谷镰孢菌牙签接种4叶期的玉米幼苗,7 d后调查接种植株,发现植株有枯萎症状和正常生长2种类型。通过特异引物对不同症状表现的植株进行PCR检测,结果表明,只有表现枯萎症状的植株体内能够检测到禾谷镰孢菌,苗期接种后仍正常生长的植株茎秆内无禾谷镰孢菌的存在。 In this study, four-leaf stage maize seedlings were inoculated with Fusarium graminearum inoculated with a high susceptible maize stalk rot 474 to inoculate the plants. After 7 days of inoculation, the plants were withered and their normal growth was observed. The results of PCR showed that Fusarium graminearum could be detected only in plants expressing wilt symptoms and that Fusarium graminearum was not found in the stalks of the plants which grew normally after seedling inoculation The presence.