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目的 研究白细胞呼吸暴发过程化学发光活性及其细胞流变学行为变化特征。方法 体外循环冠脉搭桥病人 2 0例 ,采集转流前升主动脉血样 ,分离白细胞加入FMP激活剂 ,孵化后置超弱化学发光系统中 ,计算机描记白细胞呼吸暴发化学发光性。根据发光曲线分为 5个时段 :0 =未活化 ,1=活化初期 ,2 =活化极期 ,3=活化后期 ,4=失活化 (图 1)。同步用布氏显微镜观察 (放大 10 0 0倍 ) ,分析细胞形态行为变化特征 ,按这 5个时段分为 :A、B、C、D和E型 5个细胞亚型 (图 2 ) ,并作形态、功能相关分析。结果 1 FMLP激活白细胞呼吸暴发化学发光计数峰值明显升高 ,显示白细胞氧化暴发释放反应氧类活性明显增加。 2 白细胞活化的极期、后期发光活性与形态活化率呈正相关 (r=0 .91,0 .75 ) ,并与C型、D型细胞形态改变相一致。但活化初期B型细胞因缺乏脱颗粒释放 ,未能与发光计数平行上升 ,A型和E型细胞分别反映未活化和失活化状态均相符合。结论 FMLP激活白细胞呼吸暴发化学发光活怀明显升高 ,表明白细胞释放自由基增加。布氏显微镜下细胞形态学可以区别白细胞未活化、活化和失活功能状态 ,能为临床了解白细胞活性程度提供快速诊断新方法。
Objective To study the chemiluminescence activity of leukocyte respiratory burst and the changes of its cellular rheological behavior. Methods Twenty patients with coronary artery bypass grafting underwent extracorporeal coronary artery bypass grafting. The ascending aorta blood samples were collected before the bypass and the FMP activator was added to the leukocytes. The chemiluminescence of leukocyte respiration was observed in the post-hatch ultra-weak chemiluminescence system. According to the luminous curve is divided into five periods: 0 = unactivated, 1 = early activation, 2 = activation period, 3 = activation late, 4 = inactivation (Figure 1). Simultaneously observed with a Brookfield microscope (magnification of 100 times magnification), the morphological changes of the cells were analyzed. The five time periods were divided into 5 subtypes of A, B, C, D and E types (Fig. 2) For morphology, functional correlation analysis. Results The peak value of chemiluminescence counting of FMLP-activated leukocyte respiration increased obviously, which showed that the leukocyte oxidative burst release reaction oxygen activity increased significantly. 2 Leukocyte activation at the extreme and post-luminescence activities was positively correlated with morphological activation rate (r = 0.91,0.75), and consistent with the morphological changes of C-type and D-type cells. However, in the early stage of activation, the type-B cells failed to release in parallel due to the lack of degranulation, and the type A and type E cells reflected the unactivated and inactivated states, respectively. Conclusion The chemiluminescence activity of FMLP-activated leukocyte respiration increased obviously, indicating that the leukocyte release of free radicals increased. Bromus microscope morphology of cells can distinguish between leukocyte non-activation, activation and inactivation of functional status, for clinical understanding of the degree of leukocyte activity to provide a new method for rapid diagnosis.