小棘青霉３１４９菌株的孢子经海藻酸钙包埋后用如下培养基培养：葡萄糖５．Ｏ％；蛋白胨２．Ｏ％；酵母膏０．３％；麦芽浸膏５．０％；（ＮＨ４）２ＳＯ４０．１％；ＭｇＳＯ４·７Ｈ２Ｏ０．１％；ＦｅＳＯ４·７Ｈ２０．０５％；ＮａＣ１０．５％（ｐＨ６．５），控制培养温度为２８℃，当顺式丙烯磷酸加入量为０．３％时，振荡培养８天其转化率达４０％，在同样条件下高于游离菌丝细胞，固定化孢子的代谢曲线与游离菌丝细胞相比均拖后一段时期，固定化小球可以重复使用，随着重复次数的增加，其发酵周期可由８天缩至６天。 Spore of Penicillium sp. 3149 strain was embedded in calcium alginate and cultured in the following medium: glucose 5. O%; peptone 2. Yeast extract 0.3%, malt extract 5.0%, (NH4) 2SO40.1%, MgSO4.7H2O 0.1%, FeSO4.7H2O 0.05%, NaC 10.5% (pH 6.5), Under the same conditions, the culture temperature was 28 ℃. When the content of cis-propylene phosphate was 0.3%, the conversion rate reached 40% under shaking culture for 8 days, which was higher than that of free mycelium under the same conditions. The metabolic curve of immobilized spores Free mycelial cells were delayed after a period of time, the immobilized beads can be reused, with the increase in the number of repetitions, the fermentation cycle can be reduced from 8 days to 6 days.