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以复方中药为发酵基质,米曲霉、枯草芽孢杆菌、酿酒酵母和嗜酸乳杆菌作为复合发酵菌种,纤维素酶活力和总菌数为指标,通过单因素试验和正交试验的方法,探讨多菌种混合发酵复方中药的最佳工艺条件,并测定中药及不同比例发酵中药对嗜水气单胞菌引起的澎泽鲫出血病的防治效果。结果表明:固态发酵中药的最佳工艺参数为,发酵时间72 h,初始含水量60%,总接种量9%,纤维素酶活力达到15.02 U/g。饲喂添加2%中药的饲料后,预防组澎泽鲫成活率提高52.5%,治疗组提高35%,相比较等添加量的未发酵中药,发酵中药预防组成活率提高22.5%,治疗组提高30%,发酵中药和中药对嗜水气单胞菌引起的鲫鱼出血病均有明显的预防和治疗作用(P<0.05),且发酵中药使用的效果优于未发酵中药。
Compound Chinese traditional medicine as fermentation substrate, Aspergillus oryzae, Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus acidophilus as compound strains, cellulase activity and total bacteria as indicators, by single factor test and orthogonal test method Multi-bacteria mixed fermentation of the best Chinese herbal medicine process conditions, and determine the Chinese medicine and different proportions of Chinese herbal medicine to Aeromonas hydrophila caused by the prevention and treatment of Hemorrhagic disease Peng carp crucian carp. The results showed that the optimal parameters of solid state fermentation were as follows: the fermentation time was 72 h, the initial water content was 60%, the total inoculation amount was 9% and the cellulase activity was 15.02 U / g. Compared with the same amount of unfermented Chinese medicine, the survival rate of the preventive group increased by 52.5% and that of the untreated group by 22.5% compared with that of the other groups. The survival rate of the treatment group increased 30%, fermentation of traditional Chinese medicine and Chinese medicine on Aeromonas hydrophila caused by carp bleeding have significant preventive and therapeutic effects (P <0.05), and the use of fermented Chinese medicine is better than the unfermented Chinese medicine.