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抗病转录因子Pti4是从番茄中分离的一种能够调控番茄抗病基因Pto转录的蛋白质。通过根癌农杆菌介导法将抗病转录因子基因Pti4导入花椰菜无菌苗下胚轴,同时对转基因植株进行了PCR、PCR-Southern和Southern blot分子检测以及细菌性黑腐病和软腐病病菌接种试验。结果表明,在建立再生系统过程中外植体的最佳苗龄为5~7d,培养基中激素组合为NAA0.2mg·L-1、6-BA2.0mg·L-1时不定芽的诱导和分化效果最好。获得的35株阳性植株经分子检测证明抗病转录因子基因Pti4已被整合到花椰菜的基因组中。对其中26株接种病菌发现Pti4转化植株对细菌病害具有一定的抗性。
Resistant transcription factor Pti4 is a protein isolated from tomato that regulates Pto transcription of the resistance gene in tomato. The transgenic plant Pti4 was introduced into hypocotyls of cauliflower by Agrobacterium tumefaciens-mediated method. PCR, Southern blot and Southern blot were also performed on the transgenic plants. Bacterial black rot and soft rot Pathogen inoculation test. The results showed that the best seedling age of explants during the establishment of regeneration system was 5 ~ 7d, and the induction of adventitious buds with hormone combinations of NAA0.2mg · L-1,6-BA2.0mg · L-1 The best differentiation. The 35 positive plants obtained were molecularly tested to prove that the disease-resistant transcription factor gene Pti4 has been integrated into the genome of cauliflower. Among 26 inoculated pathogens, Pti4 transformed plants were found to have some resistance to bacterial diseases.