流式细胞仪因能快速、多参数分析细胞特征,近年来在临床实验室得到广泛应用,但用于病毒检测报道不多。利用核酸特异性染料SYBR Green Ⅰ,有学者曾用流式细胞仪从海水中检测并定量病毒PpV-01。但目前仍不知流式细胞仪能否适用于所有病毒,也不知荧光强度与基因大小有无线形关系,本文作者对此进行了探讨。 Due to the rapid and multi-parameter analysis of cell characteristics, flow cytometry has been widely used in clinical laboratories in recent years, but few have been reported for virus detection. Using nucleic acid-specific dye SYBR Green I, some researchers have used flow cytometry to detect and quantitate virus PpV-01 from seawater. However, we still do not know whether FCM can be applied to all viruses, nor do we know whether there is a linear relationship between fluorescence intensity and gene size. The author of this paper discussed this.