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在临床实验室中,样品的制备技术,是成功的应用高效液相色谱法(HPLC)的一项具有决定性意义的组成部分。临床样品的直接注射,在限定的病例中虽是允许的,但通常情况下都需先进行样品的制备。理想的样品制备方法,是在除去干扰物的同时,能使待分析物得到选择性的分离。这样,既可提高分析的速度、灵敏度与准确度,也能延长分析柱的使用寿命。样品制备的方法临床样品通常为血液(全血、红细胞、白细胞、淋巴细胞、血清或血浆)和尿液。不过脑脊髓液(CSF)、羊水、胆汁、粪便与组织的活检,也都可用来进行分析。至于选择何种样品制备方法,须视样品的来源、
In clinical laboratories, sample preparation techniques are a decisive component of the successful application of high performance liquid chromatography (HPLC). Direct injection of clinical samples, although allowed in defined cases, usually requires sample preparation. The ideal method for sample preparation is to remove the interfering substances while allowing the analytes to be selectively separated. This increases the speed, sensitivity, and accuracy of your analysis, as well as extending the life of the analytical column. Methods for Sample Preparation Clinical samples are typically blood (whole blood, red blood cells, leukocytes, lymphocytes, serum or plasma) and urine. However, biopsy of cerebrospinal fluid (CSF), amniotic fluid, bile, faeces and tissue can also be used for analysis. As for the choice of what sample preparation methods, depending on the source of the sample,