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白细胞介素-2(IL-2)与其受体相互作用,可促进已活化的T 细胞生长,但只当IL-2与高亲和性受体结合才能释放与生长相关的传导信号。作者从正常人用合成的寡聚核苷酸探针筛选出编码IL-2受体特异的基因克隆。筛选出的数个阳性克隆中,P~(IL-2R)-6克隆经分析发现能编码成人T 细胞白血病细胞表达的IL-2受体的全部序列。作者为了使IL-2受体cDNA 在哺乳细胞中稳定表达,构建了一个P~(SVIL-2R)-3质粒,并
Interleukin-2 (IL-2) interacts with its receptor to promote the growth of activated T cells, but only when IL-2 binds to high-affinity receptors to release growth-related signaling. The authors screened for gene clones encoding IL-2 receptor-specific genes from normal, synthetic oligonucleotide probes. Of the several positive clones selected, the P ~ (IL-2R) -6 clone was found to encode the entire sequence encoding the IL-2 receptor expressed by adult T-cell leukemia cells. In order to stably express the IL-2 receptor cDNA in mammalian cells, a P ~ (SVIL-2R) -3 plasmid was constructed and