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目的观察利福平(RFP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致帕金森病(PD) C57BL小鼠模型的神经保护作用。方法用MPTP建立C57BL小鼠PD模型,在应用MPTP前或后给予RFP,通过行为学观察、Nissl染色、免疫组织化学染色,观察RFP对PD小鼠模型的行为学表现及黑质致密部(SNc)Nissl、TH、Bcl-2、caspase-3阳性细胞的影响。结果小鼠注射MPTP后出现震颤、竖尾、竖毛、运动迟缓、步态不稳、肢体僵硬,活动明显减少,同时SNc的Nissl、TH阳性细胞明显减少,Bcl-2阳性细胞有所增多,caspase-3阳性细胞明显增多；应用RFP后较MPTP组症状减轻,Nissl、TH、Bcl-2阳性细胞增多．而caspase-3阳性细胞减少；预使用RFP组作用最为明显。结论RFP对MPTP所致的PD小鼠中脑多巴胺能神经元凋亡具有保护作用。
Objective To observe the neuroprotective effects of rifampicin (RFP) on Parkinson’s disease (PD) C57BL mice induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) Methods PD models of C57BL mice were established by MPTP. RFP was administered before and after MPTP. Behavioral findings, Nissl staining and immunohistochemical staining were used to observe the effects of RFP on PD mouse models and SNc ) Nissl, TH, Bcl-2, caspase-3 positive cells. Results Mice treated with MPTP showed tremor, vertical tail, vertical hair, slow movement, unstable gait, stiff limbs and decreased activity. In addition, Nissl and TH positive cells were significantly decreased and Bcl-2 positive cells were increased, The number of positive caspase-3 cells increased obviously. Compared with MPTP group, the symptoms of MPTP group were alleviated and the number of Nissl, TH and Bcl-2 positive cells increased after RFP. While the number of caspase-3 positive cells decreased; the effect of pre-use RFP group was the most obvious. Conclusion RFP has a protective effect on MPTP-induced apoptosis of dopaminergic neurons in the midbrain of PD mice.