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目的:探讨葡萄糖对丁酸钠诱导的结肠癌细胞增殖抑制和凋亡效应的影响,并探讨其可能机制。方法:用MTT法检测细胞存活率。细胞凋亡用TUNEL法检测。用RT-PCR法检测葡萄糖转运蛋白1(GLUT1)、单羧酸载体1(MCT1)的mRNA表达水平。结果:低浓度葡萄糖诱导HT-29细胞系凋亡、调节其增殖,且葡萄糖能够明显抵制丁酸钠诱导凋亡和增殖抑制作用,同时对GLUT1 mRNA、MCT1 mRNA也有一定抑制作用。结论:葡萄糖浓度变化能够明显影响丁酸钠的诱导凋亡和增殖抑制作用,这种影响可能与细胞内葡萄糖和丁酸钠的浓度有关。
Objective: To investigate the effect of glucose on the proliferation and apoptosis of sodium butyrate-induced colon cancer cells and to explore its possible mechanism. Methods: Cell viability was detected by MTT assay. Apoptosis was detected by TUNEL method. The mRNA expression levels of glucose transporter 1 (GLUT1) and monocarboxylate carrier 1 (MCT1) were detected by RT-PCR. Results: Glucose could significantly inhibit the apoptosis and proliferation of HT-29 cells induced by low concentration of glucose, and inhibited the proliferation of HT-29 cells. Glucose also inhibited the expression of GLUT1 mRNA and MCT1 mRNA. CONCLUSION: The changes of glucose concentration can significantly affect the induction of apoptosis and proliferation inhibition of sodium butyrate, which may be related to the intracellular concentrations of glucose and sodium butyrate.