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本文探讨了免疫组织化学与阿片受体放射自显影相结合的方法。在相邻切片上同时观察P物质免疫组织化学和阿片受体放射自显影定位,并观察了背根切断及辣椒素处理后对大鼠脊髓内SP—免疫反应(SP—IR)及阿片受体分布的影响。结果发现:脑组织经1％甲醛固定12小时,~3H—ctorphine结合的最大结合容量及亲和常数基本上不受影响,自显影发现固定后脊髓内阿片受体(银粒)分布特异,未出现正、负
This article explores the combination of immunohistochemistry and opioid receptor autoradiography. Immunohistochemistry and opioid receptor P localization were observed simultaneously on adjacent sections. The effects of SP-IR and opioid receptors on the spinal cord of rats were observed after dorsal rhizotomy and capsaicin treatment. The impact of distribution. The results showed that the maximal binding capacity and affinity constants of ~ 3H-ctorphine binding were not affected after fixation of brain tissue with 1% formaldehyde for 12 hours. The distribution of opioid receptors (silver particles) in the spinal cord after fixation was found to be specific Appear positive, negative