实验对１３天～１５天胎龄的Ｗｉｓｔｅｒ大鼠胚胎脑组织进行深低温（－１９６℃）冷冻保存研究。根据冷冻保护剂二甲基亚砜的浓度不同分０．７Ｍ、１．５Ｍ、２．１Ｍ和２．８Ｍ四组，又根据冷冻速度分慢速和快速两种。结果随着二甲基亚砜（ＤＭＳＯ）的浓度升高冷冻存活率增高，到２．１Ｍ时为９０．１９％，但再增高时冷冻存活率提高不明显。慢速冷冻比快速存活率高。结果提示以慢速冷冻ＤＭＳＯ为２．１Ｍ进行冷冻最好 Experiments of 13 days to 15 days gestational age Wister rat embryonic brain tissue cryopreservation (-196 ℃) cryopreservation study. According to the concentration of cryoprotectant dimethyl sulfoxide in different sub-0.7M, 1.5M, 2.1M and 2.8M four groups, but also according to the freezing speed sub-slow and fast two. Results As the DMSO concentration increased, the freezing survival rate increased to 90.19% at 2.1M, but the freezing survival rate did not increase obviously when the DMSO concentration increased. Slow freezing faster than high survival rate. The results suggest that it is best to freeze at a slow freezing DMSO of 2.1M