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目的:建立体外扩增系统性红斑狼疮(SLE)患者外周血γδT细胞的方法,并初步分析其表型和功能,探索γδT细胞在SLE发病中的作用。方法:采用单克隆抗体固相法,对15例SLE患者和8例正常人外周血γδT细胞进行体外扩增建系,以流式细胞仪检测γδT细胞表型,并用MTT法观察γδT细胞对Daud i细胞的细胞毒作用。结果:建立了SLE患者外周血γδT细胞系,其平均纯度为(58.1±11.2)%,较正常对照组(80.3±9.2)%偏低(P<0.05);其细胞表型为:Vδ1(34.4±24.5)%、Vδ2(61.9±28.6)%、Vδ3(16.1±10.6)%、Vγ9(76.4±11.8)%,其中Vδ2表达较对照组降低,而Vδ1和Vδ3表达增加(P均<0.05);其细胞毒作用在二组间无明显差别。结论:SLE患者外周血γδT细胞的Vδ、Vγ基因的取用表达存在差异,提示其在SLE的发病中可能起一定作用。
OBJECTIVE: To establish a method for the amplification of γδT cells in peripheral blood of patients with systemic lupus erythematosus (SLE) in vitro and to analyze the phenotype and function of γδT cells in vitro and to explore the role of γδT cells in the pathogenesis of SLE. Methods: Monoclonal antibody solid phase method was used to amplify γδT cells in peripheral blood of 15 SLE patients and 8 normal individuals. The phenotype of γδT cells was detected by flow cytometry. The effects of γδT cells on Daud i cell cytotoxicity. RESULTS: The mean γδT cell line in peripheral blood of patients with SLE was (58.1 ± 11.2)%, which was lower than that in the normal control group (80.3 ± 9.2)% (P <0.05). The cell phenotype was Vδ1 (34.4 Vδ2 (61.9 ± 28.6)%, Vδ3 (16.1 ± 10.6)% and Vγ9 (76.4 ± 11.8)%, respectively. The expression of Vδ2 was decreased, while the expression of Vδ1 and Vδ3 was increased (all P <0.05) The cytotoxic effect was no significant difference between the two groups. CONCLUSIONS: The expression of Vδ and Vγ genes in peripheral blood γδT cells of SLE patients is different, suggesting that they may play a role in the pathogenesis of SLE.