本文报道一株能产生B细胞生长因子(BCGF)的T-T杂交瘤.实验选择5种耐受6-硫鸟嘌呤或8-氮鸟嘌呤而对氨基喋呤敏感的传代T淋巴样细胞系(CEM TG Ell、CEMF3918、MOLT-4、Jurkat 1和Jurkat F3250)供细胞融合用,用于融合的T淋巴细胞得自正常供体外周血,用10%胎牛血清(FCS)-RPMI配成1×10~6/ml的细胞悬液,加最终浓度为0.75%的植物血凝素(PHA-M),置37℃、5%CO_2中温育24～72小时,继将PHA刺激的正常T细胞与上述传代T细胞按5∶1的比例混合,融合剂用50%PEG3500.在融合5周后 This article reports a TT hybridoma that produces B-cell growth factor (BCGF). Experimentally selected five kinds of passaged T-lymphoid cell lines that are resistant to 6-thioguanine or 8-azaguanine and sensitive to aminoguanine (CEM TG Ell, CEMF3918, MOLT-4, Jurkat 1 and Jurkat F3250) for cell fusion, fused T lymphocytes from normal donor peripheral blood, 1× fetal bovine serum (FCS)-RPMI 1× 10~6/ml cell suspension plus phytohemagglutinin (PHA-M) at a final concentration of 0.75% was incubated at 37°C, 5% CO 2 for 24 to 72 hours, followed by stimulation of normal T cells stimulated by PHA. The above passaged T cells were mixed in a ratio of 5:1 and the fusion agent was 50% PEG 3500. After 5 weeks of fusion