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作者利用饱和硫酸铵沉淀和ＤＥＡＥ５２离子层析柱纯化本中心自制结肠癌单克隆抗体ＭＣ３，以此为配基与Ｓｅｐｈａｒｏｓｅ４Ｂ偶联，经免疫亲和层析从自制可溶性结肠癌抽提液中纯化出一种新的肠癌肿瘤相关抗原ＭＣ３－Ａｇ。其特性分析表明，ＭＣ３－Ａｇ可为高碘酸氧化或强硷水解，而对胰蛋白酶、神经氨酸酶、去糖酯混合液及弱硷处理不敏感，提示其决定簇可能位于Ｎ糖苷键型糖蛋白糖链上。ＳＤＳ－ＰＡＣＥ及免疫印迹分析表明ＭＣ３－Ａｇ的分子量为２８．８ｋＤ。免疫反应分析ＭＣ３－Ａｇ不与ＣＥＡ、ＡＦＰ等已知结肠癌相关抗原的单克隆抗体反应。
The authors used saturated ammonium sulfate precipitation and DEAE52 ion chromatography to purify the MC3, a home-made colon cancer monoclonal antibody, and used it as a ligand for coupling with Sepharose 4B, and purified from self-made soluble colon cancer extract by immunoaffinity chromatography. A new colorectal tumor-associated antigen, MC3-Ag. The analysis of its characteristics showed that MC3-Ag can be hydroiodic acid or strong hydrazine hydrolyzed, while it is not sensitive to trypsin, neuraminidase, deglycosylation mixture and weak cockroach treatment, suggesting that its determinants may be located in the N glycosidic bond. Type glycoproteins on the sugar chain. SDS-PACE and immunoblot analysis showed that the molecular weight of MC3-Ag was 28.8 kD. Immune Response Analysis MC3-Ag does not react with monoclonal antibodies against known colon cancer-related antigens such as CEA and AFP.