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本文用2.2M蔗糖液一次超速离心获得纯净的脑细胞精核。脑细胞核的产率为51.47%。兔脑染色质中蛋白质组份用0.14M氯化钠、0.2N盐酸、酸性氯仿—甲醇及苯酚分别抽提,可获得0.14M氯化钠可溶性核蛋白、组蛋白、非组蛋白及磷酸化非组蛋白。脑组织中非组蛋白、非组蛋白/DNA比率均低于肝脏组织。这种差异可能说明染色质非组蛋白的器官特异性。
This article uses a 2.2M sucrose solution for one-time ultracentrifugation to obtain pure brain cell sperm nuclei. The brain cell nuclei yielded 51.47%. The protein components of rabbit brain chromatin were extracted with 0.14 M sodium chloride, 0.2 N hydrochloric acid, acid chloroform-methanol, and phenol to obtain 0.14 M sodium chloride soluble nuclear protein, histone, non-histone, and phosphorylated non-protein. Histone. The ratio of non-histone and non-histone/DNA in brain tissue was lower than that in liver tissue. This difference may explain the organ specificity of chromatin non-histone.