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以分株紫萁为试验材料,采用设置不同条件(营养基质、温度和光照)或浓度(KH2PO4、激素、水分、ABA)的方法,初步探讨了分株紫萁孢子萌发、配子体发育和诱导孢子体形成的培养条件,为其孢子人工快速繁殖提供理论依据。结果表明:以草炭土与保护地土1∶1混合为育苗基质,100℃高温灭菌后播孢,保持自然变温和昼夜交替光照条件;播孢3d后,每隔3d用喷雾法喷施1次50mg/L 2,4-D,连续喷3次;每隔3d用喷雾法喷施1次0.3%KH2PO4,连续喷5次,有利于孢子萌发和配子体发育。在配子体肉眼可见时,选取生长良好的配子体进行移栽,将基质含水量调到田间持水量,移栽1周后,每隔3d用喷雾法喷施1次1.0mg/L激素ABA,连续3次,有利于配子体形成孢子体和生长发育。
Using ramets as the material, the germination of gametophyte, the development of gametophyte and the induction of spores were preliminarily studied by setting different conditions (nutrient matrix, temperature and light) or concentration (KH2PO4, hormone, Body formation of culture conditions for artificial spores rapid propagation provide a theoretical basis. The results showed that: the mixture of peat soil and protective soil 1: 1 as seedling substrate, 100 ℃ high temperature sterilization after sowing, to maintain the natural temperature and alternating day and night light conditions; 3d Spraying Spraying 3d Times 50mg / L 2,4-D, spraying 3 times in a row; spraying 0.3% KH2PO4 once every 3 days by spray method, spraying 5 times in a row, which is good for spore germination and gametophyte development. When the gametophyte is visible to the naked eye, the well-grown gametophyte is selected for transplanting, and the substrate water content is adjusted to the field water holding capacity. After transplanting for 1 week, 1.0 mg / L hormone ABA is sprayed once every 3 days by spraying. Times, is conducive to the formation of the gametophyte sporophyte and growth and development.