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目的研究不同浓度1α,25-(OH)2D3(0、10-11、10-9、10-7 mol/L)对体外培养成骨细胞(osteoblasts,OB)内游离钙离子浓度([Ca2+]i)的影响及钙离子通道机制。方法在体外培养SD大鼠OB基础上,添加不同浓度的1α,25-(OH)2D3或/和10-8 mol/L硝苯地平(NIF)作用20 min,流式细胞仪测定[Ca2+]i。结果 10-11 mol/L 1α,25-(OH)2D3组[Ca2+]i显著低于对照组(P<0.05),10-9、10-7 mol/L组[Ca2+]i显著或极显著高于对照组(P<0.05或P<0.01);单独添加10-8 mol/L NIF组[Ca2+]i与对照组差异不显著(P>0.05);联合添加10-9 mol/L 1α,25-(OH)2D3和10-8 mol/L NIF组[Ca2+]i与对照组差异不显著(P>0.05),但显著低于单独添加10-9 mol/L 1α,25-(OH)2D3组(P<0.05)。结论 1α,25-(OH)2D3引起[Ca2+]i改变的过程涉及L-型钙离子通道。
Objective To study the effects of different concentrations of 1α, 25- (OH) 2D3 (0,10-11,10-9,10-7 mol / L) on the intracellular free calcium concentration ([Ca2 +]) in cultured osteoblasts i) and calcium channel mechanism. Methods Sprague-Dawley rats were cultured with different concentrations of 1α, 25- (OH) 2D3 or / and 10-8 mol / L Nifedipine (NIF) for 20 min in vitro. Flow cytometry was used to determine the [Ca2 + i. Results [Ca2 +] i in 10-11 mol / L 1α, 25- (OH) 2D3 group was significantly lower than that in control group (P <0.05), and [Ca2 +] i in 10-9,10-7 mol / L group was significantly or extremely significant (P <0.05 or P <0.01). Compared with the control group, the Ca2 + concentration of 10-8 mol / L NIF alone group was not significantly different (P> 0.05) There was no significant difference between the control group and the 25- (OH) 2D3 group and the 10-8 mol / L NIF group (P> 0.05) 2D3 group (P <0.05). Conclusions The process of [Ca2 +] i change induced by 1α, 25- (OH) 2D3 involves L-type calcium channel.