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［目的］建立一种简便、实用的检测大量水样中的微量脊髓灰质炎病毒的方法。［方法］将Ｌ２０Ｂ细胞 加入大量的含微量脊髓灰质炎病毒的水中，充分作用后收集细胞，作病毒分离培养。同时以 ＮａＣ１—Ａ１Ｃ１_３沉淀法作对照。 ［结果］１０００ｍｌ水样中含病毒１００、１０ＴＣＤ_５０时能全部检出：当合１～２ＴＣＤ_５０时１４份样本可检出１３份。ＮＳＣ１—Ａ１Ｃ１_３ 沉淀法在１００ＴＣＤ_５０时４份样本中仅检出１份，其它各浓度的４份样都未能检出。
[Objective] To establish a simple and practical method for detecting trace poliovirus in a large number of water samples. [Method] L20B cells were added to a large amount of poliovirus-containing water, and the cells were harvested after full effect and were isolated and cultured as viruses. At the same time NaC1-A1C1_3 precipitation method for the control. [Result] The results showed that when 1000ml water samples contained virus 100 and 10TCD_50 were all detected, 13 samples could be detected in 14 samples when combined with 1 ~ 2TCD_50. NSC1-A1C1_3 precipitation method in the 100TCD_50 4 samples were only detected in 1, the other concentrations of 4 samples were not detected.