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以伯氏疟原虫K_(173)株,小鼠体内试验系统,采用剂量递增连续3天给药法,历时420天,连续血传60代,培育出高度抗氯喹的RC/k173系,其抗性指数大于100.以维持剂量继续血传60代或经冷冻保存360天,其抗性强度仍保持在原来水平.但停药血传到第10代,抗性基本消失.抗药系原虫的毒力较亲代系低.对7种常用抗疟药敏感性测试的结果表明,该系除对乙胺嘧啶、周效磺胺和伯喹敏感外,对另外4种药物均显示出程度不等的交叉抗药性.推荐该品系可作为新抗疟化合物与氯喹交叉抗性研究的模型,并以给药血传或冷冻保存作为保种方法.
The Plasmodium berghei K_ (173) strain and mouse in vivo test system were administered continuously for 3 days with dose increasing for 420 days. Sex index is greater than 100. To maintain the dose of heredity 60 generations or cryopreservation 360 days, the resistance intensity remained at the original level.But stopping the blood passed to the 10th generation, the resistance basically disappeared. The virulence was lower than that of the parental strain.The results of the sensitivity tests on seven commonly used antimalarial drugs showed that the strains showed different degrees of sensitivity to the other four drugs in addition to pyrimethamine, pesosin and primaquine It is recommended that the strain be used as a model for the cross resistance study of new antimalarial compounds with chloroquine and as a conservation method by blood donation or cryopreservation.