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近来一些研究证明,在内脏大神经末梢,有阿片肽与乙酰胆硷(ACh)同时存在。阿片肽可作为辅递质,对突触后膜上的ACh受体起调制作用。但是,在中枢神经系统内,阿片类物质能否影响M-胆硷能受体,迄今尚未见报告。本工作应用~3H-QNB(二苯羟乙酸奎宁酯)放射受体分析法(Yamamura,1974),研究了吗啡类药物对中枢M-胆硷能受体亲和力K_D和总结合力(R_O,受体分子数目)的影响。 M-胆硷能受体蛋白是取大白鼠全脑(去小脑),以0.32M蔗糖液(1:10w/v)制成匀浆,经冷冻差速离心(1,000g15’,48,000g 45’和20,000g30’)分离提取。调受体蛋白浓度至5毫克/毫升。
Recently, some studies have shown that in the visceral large nerve endings, opioid peptide and acetylcholine (ACh) co-exist. Opioid peptide can be used as a secondary neurotransmitter ACh receptor on the postsynaptic membrane modulating effect. However, in the central nervous system, opioids can affect the M-cholinergic receptors, so far no report. In this work, ~ 3H-QNB (quinine diphenylacetate) radioimmunoassay (Yamamura, 1974) was used to study the effect of morphine on the central M-cholinergic receptor affinity K_D and total binding force The number of body molecules). The M-choline receptor protein was homogenized in whole brain (to the cerebellum) of rats and homogenized in 0.32 M sucrose solution (1: 10 w / v) and subjected to freeze-centrifugation (1,000 g of 15 ’, 48,000 g of 45’ And 20,000g30 ’). Adjust receptor protein concentration to 5 mg / mL.