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目的探讨血管内皮损伤在外膜损伤致内膜病变中的作用。方法选用纯种新西兰大白兔,采用胶原酶消化+机械分离的方法建立外膜损伤动物模型,HE染色后,观察外膜损伤血管的形态变化。采用Medlab-U/4C501生物信号采集处理系统检测血管内皮依赖性舒张功能,电镜下观察血管内皮细胞的微观形态变化。用实时PCR检测单核细胞趋化蛋白-1(MCP-1)mRNA的表达。结果外膜损伤后3 d血管内皮依赖性舒张功能减退及内皮细胞损伤,7 d出现内膜增生性病变。结论血管外膜损伤可能通过损伤血管内皮导致内膜病变的形成。
Objective To investigate the role of vascular endothelial injury in endometrial lesions caused by adventitial injury. Methods Pure New Zealand white rabbits were selected. Animal models of adventitial injury were established by collagenase digestion and mechanical separation. After HE staining, morphological changes of blood vessels were observed. Medlab-U / 4C501 biological signal acquisition and processing system was used to detect endothelium-dependent vasodilatation, and the morphological changes of vascular endothelial cells were observed under electron microscope. The expression of monocyte chemotactic protein-1 (MCP-1) mRNA was detected by real-time PCR. Results Endothelium-dependent vasodilation and endothelial cell injury were observed 3 days after injury of outer membrane, and neointimal hyperplasia occurred on the 7th day. Conclusion Vascular adventitial injury may lead to the formation of endometrial lesions through vascular endothelial injury.