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目的监测了解主要食品中致病菌污染状况,构建广西食品致病菌污染数据库,为有效防治食源性疾病提供科学依据。方法在南宁、桂林等6个市采集6大类食品,按国家标准或国际标准方法对食品中沙门氏菌、单核细胞增生李斯特菌(L.m)、大肠杆菌O157:H7、志贺氏菌以及海产品中的副溶血弧菌进行分离与鉴定。结果2002~2004年在6市5类食品中检出沙门氏菌54株,检出率4.49%;其次为检出单增细胞李斯特菌51株,检出率为4.24%,志贺氏菌为1.64%、大肠杆菌O157:H7为0.25%;而另1类食品海产品中副溶血检出率高达20.00%(41/205)。结论广西主要食品均遭受食源性致病菌不同程度的污染;其中以生肉污染最为严重,受到了沙门氏菌、L.m、O157:H7、志贺氏菌的污染,熟肉、水产品、生食蔬菜也遭受L.m污染;而海产品副溶血弧菌污染严重。有针对性防范和控制各类食品致病菌的污染,是防止食源性疾病暴发的重要措施。
Objective To monitor and understand the status of pathogen contamination in major foodstuffs and establish a database of foodborne pathogen contamination in Guangxi so as to provide a scientific basis for the effective prevention and treatment of foodborne diseases. Methods Six kinds of foodstuffs were collected in six cities such as Nanning and Guilin. According to the national standard or the international standard method, Salmonella, Listeria monocytogenes (Lm), Escherichia coli O157: H7, Shigella and sea Vibrio parahaemolyticus in the product was isolated and identified. Results From 2002 to 2004, 54 strains of Salmonella were detected in 5 kinds of food in 6 cities, with a detection rate of 4.49%; followed by detection of 51 strains of Listeria monocytogenes, the detection rate was 4.24%, and Shigella was 1.64 %, Escherichia coli O157: H7 was 0.25%; while the other one type of seafood haemolymph detection rate as high as 20.00% (41/205). Conclusions The main foods in Guangxi are all contaminated by food-borne pathogens to varying degrees. Among them, the most serious raw meat is polluted by Salmonella, Lm, O157: H7 and Shigella, cooked meats, aquatic products and raw vegetables Suffer Lm pollution; and seafood parahaemolyticus contamination is serious. Targeted prevention and control of various types of foodborne pathogens contamination is an important measure to prevent foodborne disease outbreaks.