背景:通过生理和分子方法,已经验证了从火山土中提取的腐殖质在玉米幼苗氮同化中的作用。用不同浓度的腐殖质(0、1和5mg/L)处理玉米幼苗48h,在它们生长过程中,对参与氮代谢和三羧酸循环的可溶性蛋白质、氨基酸和相关酶的活性进行监测。为了更好的了解腐殖质的作用机理,在玉米幼苗生长到两周时,分别在充足氮 BACKGROUND: The physiological and molecular approaches have validated the role of humus extracted from volcanic soils in nitrogen assimilation in maize seedlings. Corn seedlings were treated with different concentrations of humus (0, 1, and 5 mg / L) for 48 h, and the activities of soluble proteins, amino acids and related enzymes involved in nitrogen metabolism and tricarboxylic acid cycling were monitored during their growth. In order to better understand the mechanism of action of humus, corn seedlings were grown to two weeks, respectively, in a sufficient nitrogen