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A novel electrochemical detection approach for platelet-derived growth factor(PDGF) via “sandwich”structure is reported in this paper. 3D-4MgCO_3 Mg(OH)_2 4H_2O-Au NPs inorganic hybrid composite was utilized as immobilized substrate for sensitive PDGF detection and Pt-Au bimetallic nanoparticles were labelled on PDGF aptamer to indirectly detect PDGF for the first time. The proposed aptasensor exhibited a high catalytic efficiency towards reduction of H_2O_2, hence the sensitive detection of PDGF was achieved.Results showed that the aptasensor exhibited excellent linear response to PDGF, in the range of 0.1 pg/m L–10 ng/m L(4 fmol/L–400 pmol/L), with detection limit of 0.03 pg/m L(1.2 fmol/L).
A novel electrochemical detection approach for platelet-derived growth factor (PDGF) via “sandwich” structure is reported in this paper. 3D-4MgCO_3 Mg (OH) _2 4H_2O-Au NPs inorganic hybrid composite was utilized as immobilized substrate for sensitive PDGF detection and Pt-Au bimetallic nanoparticles were labeled on PDGF aptamer to indirectly detect PDGF for the first time. The proposed aptasensor exhibited a high catalytic efficiency towards reduction of H_2O_2, hence the sensitive detection of PDGF was achieved. Results showed that the aptasensor enabled linear response to PDGF in the range of 0.1 pg / m L-10 ng / m L (4 fmol / L-400 pmol / L) with a detection limit of 0.03 pg / m L (1.2 fmol / L).