论文部分内容阅读
实验对分离纯化了的姥鲨软骨新生血管抑制因子 (Shark cartilage derived inhibitor,SCDI)进行了生物学活性研究 ,结果表明它对 型胶原酶、内皮细胞 DNA合成、角膜新生血管和荷瘤小鼠的肿瘤生长具有较明显的抑制作用。12 μg SCDI可竞争抑制一个单位的胶原酶活性 ;其对小鼠心肌和肺毛细血管内皮细胞 DNA合成的 IC50 分别为 3.38μg和 11.74 μg;高剂量组 (40 μg)和低剂量组 (10μg) SCDI对家兔眼角膜新生血管的抑制率分别为 70 .2 4 %和 38.38% ;SCDI在 2 0 mg/kg的剂量下对 B16黑色素瘤和 L ewis肺癌的抑瘤率分别为 4 5.4 5%和 37.84 % .
The biological activity of isolated and purified Shark cartilage derived inhibitor (SCDI) was studied. The results showed that it was effective against collagenase, endothelial cell DNA synthesis, corneal neovascularization and tumor-bearing mice. Tumor growth has a significant inhibitory effect. 12 μg of SCDI can compete to inhibit one unit of collagenase activity; its IC50 for DNA synthesis of mouse cardiac and pulmonary capillary endothelial cells is 3.38 μg and 11.74 μg, respectively; high dose (40 μg) and low dose (10 μg) The inhibitory rate of SCDI on corneal neovascularization in rabbits was 70.24% and 38.38%, respectively; the inhibitory rate of SCDI on B16 melanoma and Lewis lung cancer was 45.45% at a dose of 20 mg/kg SCDI. And 37.84 %.