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采用荧光素异硫氰酸酯 ( FITC)对新建立的荧光光纤免疫磁珠流动分析系统参数及性能进行了考察。不同泵速下测定流量的恒定性 ,其 RSD为 0 .2 3%～0 .76 % ( n=2 4 ) ;对 1× 1 0 -5～ 1× 1 0 -2 mol·L-1FITC溶液 ,线性相关系数 0 .986～ 0 .999;连续测定 1 2次 ,RSD为 0 .73%～ 0 .95% ;2 h内基线不产生飘移。
Fluorescein isothiocyanate (FITC) was used to investigate the parameters and performance of the newly established fluorescence optical fiber magnetic bead flow analysis system. The RSD was 0.23% ~ 0.76% (n = 2 4) at different pump velocities. For 1 × 10 -5 ~ 1 × 10 -2 mol·L-1 FITC solution , Linear correlation coefficient of 0.986 ~ 0.999; continuous determination of 12 times, RSD 0.73% ~ 0.95%; 2 h baseline did not drift.