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目的:建立HPLC-UV-ELSD同时测定散结镇痛胶囊中皂苷类和黄酮类成分的方法,比较15批散结镇痛胶囊中各成分含量。方法:采用HPLC-UV-ELSD联用法,Waters Symmetry@C18色谱柱(4.6 mm×250 mm,5μm),乙腈(A)-水(B)梯度洗脱(1~13 min,19%~37%A;13~50 min,37%~36%A),流速1 m L·min-1,检测波长278 nm;蒸发光散射检测器(ELSD)漂移管温度45℃,载气流速3.7 L·min-1,同时测定散结镇痛胶囊中三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1,龙血素A和龙血素B的含量。结果:建立了同时测定散结镇痛胶囊中3个皂苷类和2个黄酮类成分含量的方法,线性关系良好(0.999 5~0.999 7),平均回收率为99.1%~104.2%。结论:该方法简便,重复性好,适用于散结镇痛胶囊皂苷类和黄酮类成分的同时测定,亦适用于散结镇痛胶囊的的质量控制。
OBJECTIVE: To establish a HPLC-UV-ELSD method for the simultaneous determination of saponins and flavonoids in Sanjiezhitong Capsules and to compare the contents of the constituents in 15 batches of Sanjie Analgesic Capsules. Methods: The HPLC-UV-ELSD method was used for the gradient elution with acetonitrile (A) -water (B) on a Waters Symmetry @ C18 column (4.6 mm × 250 mm, A; 13-50 min, 37-36% A), flow rate 1 m L · min-1, detection wavelength 278 nm; evaporative light scattering detector (ELSD) drift tube temperature 45 ℃, carrier gas flow rate 3.7 L · min -1, and determine Sanjie analgesic capsule notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1, loureirin A and loureirin B content. Results: The method for simultaneous determination of three saponins and two flavonoids in Sanjiezhutong Capsules was established. The linear relationship was good (0.999 5-0.9999 7) and the average recovery was 99.1% -104.2%. Conclusion: The method is simple and reproducible. It is suitable for the simultaneous determination of saponins and flavonoids in Sanjiezhuotong Capsules. It is also suitable for the quality control of Sanjiezhuotong Capsules.