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目的 通过观察脊髓损伤动物膀胱内灌注辣椒辣素后膀胱氮能神经的变化 ,探讨氮能神经在病理性膀胱中的作用。 方法 成年健康SD大鼠 4 4只 ,随机分为正常组、假损伤组及脊髓损伤模型组。模型组横断第 8～ 9胸髓后饲养 4～ 5周。取部分模型组大鼠行膀胱内辣椒辣素 (1mmol/L)灌注。所有动物膀胱基底部、体部及顶部分别进行ncNOS免疫组化检测。统计分析采用t检验。 结果 大鼠NOS阳性神经分布较少 ,模型组 (12 .8± 1.3)与正常组 (11.1± 0 .9)比较差别无显著性意义 (P >0 .0 5 ) ,辣椒辣素灌注后膀胱NOS阳性神经分布显著增加 (2 6 .1± 2 .7,P <0 .0 5 )。 结论 辣椒辣素灌注膀胱使膀胱氮能神经分布增加 ,提示辣椒辣素的作用机理与NO有关。
OBJECTIVE: To investigate the effect of nitrogenase Nerve in pathological bladder by observing the changes of bladder Nerve after intravesical instillation of capsaicin in spinal cord injured rats. Methods Forty four adult SD rats were randomly divided into normal group, sham injury group and spinal cord injury model group. The model group was transected from 8th to 9th thoracic marrow for 4 to 5 weeks. Take part of the model group rats intravesical capsaicin (1mmol / L) perfusion. All animal bladder base, body and the top were ncNOS immunohistochemical detection. Statistical analysis using t test. Results NOS positive nerve distribution was less in model group (12.8 ± 1.3) compared with normal group (11.1 ± 0.9), there was no significant difference (P> 0.05) NOS positive nerve distribution was significantly increased (26.1 ± 2 .7, P <0.05). Conclusion Capsaicin infusion into the bladder increases the distribution of NAC in the bladder, suggesting that the mechanism of action of Capsaicin is related to NO.