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目的制定妇科止血灵中五味子醇甲的含量测定方法。方法采用高效液相色谱(HPLC)法测定妇科止血灵中五味子醇甲的含量。色谱柱为Agilent C_(18)(5μm,250 mm×4.6 mm),流动相为甲醇-水(65∶35),流速为1.0 mL/min,检测波长为250 nm,柱温为25℃;并对线性关系、精密度、稳定性、重复性等进行方法学考察。结果五味子醇甲在5.843~52.587μg/m L范围内呈良好的线性关系,回归方程为Y=2.453 9X+3.076 4,r=0.999 9;本品的平均回收率为102.02%,RSD为1.3%;五味子醇甲平均含量为59.40μg/g。结论该方法简便、灵敏、准确、重现性好、精密度高,可作为妇科止血灵中五味子醇甲的定量测定方法。
Objective To develop a method for the determination of schisandrin in gynecological hemostatic spirit. Methods HPLC method was used to determine the content of schisandrin in Gynecology. The mobile phase consisted of methanol-water (65:35), the flow rate was 1.0 mL / min, the detection wavelength was 250 nm and the column temperature was 25 ℃. The column was Agilent C 18 (5 μm, 250 mm × 4.6 mm) On the linear relationship, precision, stability, repeatability and other methodological study. Results Schisandrin A showed a good linear relationship in the range of 5.843-52.587μg / m L with a regression equation of Y = 2.453 9X + 3.076 4 and r = 0.999 9. The average recoveries of this product were 102.02% with a RSD of 1.3% ; Schisandra alcohol average content of 59.40μg / g. Conclusion The method is simple, sensitive, accurate, reproducible, high precision, and can be used as a quantitative method for the determination of Schisandrins A in gynecological hemostatic tablets.