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目的探讨人参低聚肽(GOP)对酒精诱导的大鼠急性肝损伤的保护作用及其可能机制。方法采用SPF级SD雄性大鼠,随机分为7组,每组10只,包括,1个空白对照组、1个模型对照组、1个乳清蛋白对照组(0.250 0 g/kg)和4个GOP剂量组(0.062 5、0.125 0、0.250 0、0.500 0 g/kg)。连续灌胃30d后,采用体积分数50%的酒精以7g/(kg·BW)剂量灌胃造成大鼠急性酒精性肝损伤模型,测定大鼠血清ALT、AST活性和肝脏SOD、GSH、GSH-PX及MDA含量。结果与模型对照组相比,GOP干预可以显著降低大鼠血清ALT、AST和肝组织MDA(P<0.05或P<0.01)的水平,增加肝组织SOD、GSH和GSH-PX的活性(P<0.05或P<0.01)。结论 GOP对酒精诱导引起的大鼠急性肝损伤具有保护作用,其机制可能与提高机体抗氧化水平有关。
Objective To investigate the protective effect of ginseng oligopeptide (GOP) on alcohol-induced acute liver injury in rats and its possible mechanism. Methods SPF SD male rats were randomly divided into 7 groups with 10 rats in each group, including 1 blank control group, 1 model control group, 1 whey protein control group (0.250 g / kg) and 4 GOP dose group (0.062 5, 0.125 0, 0.250 0, 0.500 0 g / kg). After 30 days of continuous gavage, the model of acute alcoholic liver injury was induced by intragastric administration of 50% ethanol (7 g / (kg · BW)), the activity of serum ALT and AST and the activity of SOD, GSH and GSH- PX and MDA content. Results Compared with the model control group, GOP treatment could significantly decrease the levels of serum ALT, AST and liver MDA (P <0.05 or P <0.01), and increase the activity of SOD, GSH and GSH-PX in liver tissue (P < 0.05 or P <0.01). Conclusion GOP has a protective effect on alcohol-induced acute liver injury in rats, and its mechanism may be related to improving the level of antioxidation.