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目的:研究土茯苓与肿节风不同比例配伍对落新妇苷含量的影响。方法:采用高效液相色谱法测定落新妇苷的含量。色谱柱为Diamonsil Cl8(2)(250 mm×4.6 mm,5μm),流动相为乙腈-0.1%冰醋酸(20:80),流速为1.0 m L·min~(-1),柱温30℃,检测波长为291 nm,进样量10μL。结果 土茯苓和肿节风配伍共同煎煮,当土茯苓和肿节风的配伍比例为1:1、3:1时,提取液中落新妇苷的含量明显增加;当土茯苓和肿节风的配伍比例为1:3时,提取液中落新妇苷的含量显著降低;其它配伍比例时,含量增加不明显。两药单煎合并也有一定的增溶作用。结论 土茯苓和肿节风配伍提取对落新妇苷的含量有一定的影响,临床使用时以1:1或3:1配伍入药为佳,尽量避免1:3配伍入药。
Objective: To study the effect of different proportions of Smilax glabra and swollen-stem-type wind on the content of astilbin. Methods: Determination of astilbin content by high performance liquid chromatography. The column was Diamonsil Cl8 (2) (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.1% glacial acetic acid (20:80) at a flow rate of 1.0 mL · L -1 , Detection wavelength of 291 nm, injection volume 10μL. The results of soil Fuling and swollen joint wind boiling and compatibility, when the Smilax glabra and swollen joint wind compatibility ratio of 1: 1, 3: 1, the content of astilbin in the extract increased significantly; when Smilax and Poria When the ratio of compatibility was 1: 3, the content of astilbin in the extract was significantly reduced; while the other compatibility ratio, the content increased not obvious. Combination of two single fried also have some solubilization. Conclusion Tuckahoe and swollen section wind compatibility extraction of astilbin content has a certain impact, clinical use of 1: 1 or 3: 1 compatibility is better medicine, try to avoid 1: 3 compatibility with medicine.