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血卟啉衍生物(HPD)光敏作用有明确的杀伤肿瘤效应,其作用机制已有不少报导;我们在研究离体人肿瘤和正常细胞对[~3H]-HPD的摄取和存留的基础上,用液闪计数及细胞计数方法,测定HPD-红光对离体人胃癌MGC80-3细胞DNA合成和细胞杀伤作用。 MGC80-3细胞用小方瓶培养,每瓶接种1.5×10~5细胞,加2ml培养液(含15％小牛血清的Eagle液),细胞在37℃温箱培养16～18小时,以5μg/mlHPD处理30分钟、红光(波长6300A±,功率密度50mw/cm~2)照射5分钟,换含[~3H]-胸苷(1μcl/ml)培养液,继续培养,用液闪计数方法,测定5和30分钟及2、6、10、24、48、72小时标本中掺入[~3H]-胸苷的cpm值。为测定HPD-红光的细胞杀伤效应,经
The photosensitization of hematoporphyrin derivatives (HPD) has a clear killing effect on tumors, and its mechanism of action has been reported; we are investigating the uptake and persistence of [~3H]-HPD in isolated human tumors and normal cells. Using liquid scintillation counting and cell counting methods, the DNA synthesis and cell killing effects of HPD-red light on human gastric cancer MGC80-3 cells were determined. MGC80-3 cells were cultured in small square flasks and inoculated with 1.5 x 10~5 cells per flask. 2 ml of culture medium (Eagle solution containing 15% calf serum) was added. The cells were incubated incubator at 37°C for 16 to 18 hours to 5 μg. /mlHPD treatment for 30 minutes, red light (wavelength 6300A ±, power density 50mw/cm~2) irradiation for 5 minutes, for containing [~ 3H]-thymidine (1μcl/ml) culture medium, continue to culture, using liquid scintillation counting method The cpm values of [~3H]-thymidine incorporated into 5, 30, and 2, 6, 10, 24, 48, and 72 hour samples were determined. To determine the cytotoxic effect of HPD-red light,