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目的:建立野豌豆药材中芦丁、金丝桃苷和槲皮素三个活性成分的HPLC含量测定方法。方法:采用Agilent ZORBAX Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈-1‰磷酸水溶液,梯度洗脱,流速:0.8 ml·min-1,检测波长:370nm,柱温:30℃。结果:芦丁、金丝桃苷和槲皮素的线性范围分别为4.090~130.940μg·ml-1(r=0.999 9)、4.600~147.200μg·ml-1(r=0.999 9)和0.810~25.780μg·ml-1(r=0.999 8);平均回收率分别为103.45%(RSD=1.25%)、98.96%(RSD=1.77%)和102.88%(RSD=0.84%)(n=6)。结论:本方法稳定,重复性好,操作简单,可用于野豌豆药材的质量控制。
Objective: To establish a HPLC method for the determination of rutin, hyperoside and quercetin in Vicia faba. Methods: The mobile phase consisted of acetonitrile-1 ‰ phosphoric acid solution with gradient elution at a flow rate of 0.8 ml · min-1 using Agilent ZORBAX Eclipse XDB-C18 column (250 mm × 4.6 mm, 5 μm) Temperature: 30 ℃. Results: The linear ranges of rutin, hyperoside and quercetin were 4.090-130.940μg · ml-1 (r = 0.999 9), 4.600-147.200μg · ml-1 (r = 0.999 9) and 0.810 ~ The average recoveries were 103.45% (RSD = 1.25%), 98.96% (RSD = 1.77%) and 102.88% (RSD = 0.84%) (n = 6) respectively. Conclusion: The method is stable, reproducible and easy to operate. It can be used for the quality control of Vicia faba.