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采用 CM—Sephadex C—50,SP—Sephadex C—25离子交换柱层析和 SephadexG—50凝胶过滤三步分离程序,从东亚钳蝎毒中得到一种新的哺乳动物毒素。经低 pH 系统不连续聚丙烯酰胺凝胶园盘电泳,SDS—不连续聚丙烯酰胺凝胶板电泳及等电聚焦聚丙烯酰胺凝胶园盘电泳鉴定说明该毒素为电泳纯的蛋白质。用 SDS 电泳法测得其分子量为8,750道尔顿,等电聚焦电泳法测定 pH 为8.2。纯化成份的产率为2.5%。
Using CM-Sephadex C-50, SP-Sephadex C-25 ion-exchange column chromatography and Sephadex G-50 gel filtration three-step separation procedure, a new mammalian toxin was obtained from toadstool poison. The low pH system discontinuity polyacrylamide gel electrophoresis, SDS-discontinuous polyacrylamide gel electrophoresis and isoelectric focusing polyacrylamide gel electrophoresis showed that the toxin was electrophoretically pure protein. The molecular weight was determined to be 8,750 daltons by SDS electrophoresis and the pH was determined to be 8.2 by isoelectric focusing electrophoresis. The yield of purified components was 2.5%.