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比利时学者Wisse等在肝原位观察和分离细胞研究中,首先发现肝血窦内存在一种未曾报道的细胞,它的特点是细胞核偏位,中央胞质内含有致密核蕊膜包颗粒,认为它可能是一种内分泌细胞,命名为“pit cell”。近些年的许多研究证实,这种细胞是定居在肝内的具有自然杀伤作用的大颗粒淋巴细胞(LGL),并已公认它是肝内的第4种非肝细胞。由于肝是机体起防御和免疫功能的一道重要防线,因此,对肝LGL免疫特性的研究颇为注意,但国内尚未有类似研究。我们为探讨肝LGL的抗肝癌作用及其与枯否细胞等的关系,首先成功地从大鼠肝分离出高纯度的LGL。
The Wisse et al. of Belgian scholars observed in situ observation and isolation of cells in the liver. Firstly, it was found that there was an unreported cell in the hepatic sinusoids. It was characterized by the eccentricity of the nucleus, and the central cytoplasm contained a dense core of nuclear envelope particles. It may be an endocrine cell, named “pit cell.” Many studies in recent years have confirmed that this cell is a natural killer large granular lymphocyte (LGL) that colonizes the liver and has been recognized as the fourth non-hepatocyte in the liver. Since the liver is an important line of defense for the defense and immune function of the body, the study on the immune characteristics of liver LGL is quite noticeable, but there is no similar research in China. To explore the anti-hepatocarcinoma effect of liver LGL and its relationship with Kupffer cells, we first successfully isolated high-purity LGL from rat liver.