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目的探讨非小细胞肺癌(NSCLC)患者共刺激分子CD28、细胞毒T淋巴细胞相关抗原4(CTLA-4)、程序性死亡分子-1(PD-1)、程序性死亡配体-1(PD-L1)mRNA的表达及意义。方法采用Taqman探针实时荧光定量PCR方法检测50例NSCLC患者、30例肺良性疾病患者及50例健康人外周血共刺激分子CD28、CTLA-4、PD-1、PD-L1 mRNA的表达水平。结果与健康人相比,NSCLC及肺良性疾病患者CD28 mRNA表达显著降低(P<0.01),PD-1 mRNA表达显著增加(P<0.01),CTLA-4 mRNA及PD-L1 mRNA表达差异无统计学意义(P>0.05),与肺良性疾病患者相比,NSCLC患者4项指标差异无统计学意义(P>0.05);Ⅲ+Ⅳ期NSCLC患者CD28 mRNA表达低于Ⅰ+Ⅱ期患者(P<0.05),有淋巴结转移的NSCLC患者CD28 mRNA表达低于无淋巴结转移的患者,PD-1 mRNA表达高于无淋巴结转移的患者(P<0.05)。结论 NSCLC患者B7-CD28家族分子表达异常是其免疫功能紊乱和疾病恶化进展的重要原因。
Objective To investigate the expression of costimulatory molecules CD28, CTLA-4, PD-1, PD-1 in patients with non-small cell lung cancer -L1) mRNA and its significance. Methods The expression of costimulatory molecules CD28, CTLA-4, PD-1 and PD-L1 in peripheral blood of 50 patients with NSCLC, 30 patients with benign lung disease and 50 healthy people were detected by Taqman real-time PCR. Results Compared with healthy controls, the expression of CD28 mRNA and the expression of PD-1 mRNA in NSCLC patients and benign lung disease patients were significantly lower than those in healthy controls (P <0.01). There was no statistical difference between CTLA-4 mRNA and PD-L1 mRNA expression (P> 0.05). Compared with patients with benign lung disease, there were no significant differences in the four indicators of NSCLC patients (P> 0.05). The expression of CD28 mRNA in patients with stage Ⅲ + Ⅳ NSCLC was lower than that in patients with stage Ⅰ + Ⅱ <0.05). The expression of CD28 mRNA in NSCLC patients with lymph node metastasis was lower than that without lymph node metastasis, and the expression of PD-1 mRNA was higher in patients with lymph node metastasis than those without lymph node metastasis (P <0.05). Conclusion Abnormal expression of B7-CD28 family members in NSCLC patients is an important reason for the progression of immune dysfunction and disease progression.