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目的:本研究采用mRNA-DD法鉴定、筛查与脊索瘤发生的相关基因,为脊索瘤的分子机制、基因诊断和基因治疗的研究提供靶基因。方法:应用mRNA-DD方法,对1例脊索瘤肿瘤和瘤旁正常组织的差异条带进行扩增、纯化、测序分析,鉴定与脊索瘤相关的基因,并应用R T-PCR方法对18例脊索瘤进行筛查。结果:mRN A-DD检测结果在1例脊索瘤中发现9条差异片段,对其中2条进行测序分析,发现分别与RNF-11和CTC-50DM5基因具有高度同源性。对全部18例脊索瘤筛查结果显示RNF-11和CTC-50DM5基因在脊索瘤和瘤周正常组织中表达差异均具有统计学意义(P<0.001),其中RNF-11基因表达在瘤周组织中明显增高,而CTC-50DM5基因表达在脊索瘤组织中明显增高。结论:RNF-11基因可能是与脊索瘤相关的肿瘤抑制基因,而CTC-50DM5基因可能是与脊索瘤相关的癌基因。
OBJECTIVE: In this study, mRNA-DD method was used to identify and screen related genes related to chordoma and to provide target genes for the molecular mechanism of chordoma, gene diagnosis and gene therapy. Methods: The differential bands of 1 chordoma tumor and adjacent normal tissue were amplified by mRNA-DD method, purified, sequenced and analyzed, and chordoma-related genes were identified. RT-PCR was used to detect 18 cases Chordomas are screened. Results: The mRN A-DD test results showed that 9 differentially expressed fragments were found in one chordoma. Two of them were sequenced and found to be highly homologous to the RNF-11 and CTC-50 DM5 genes, respectively. All 18 cases of chordoma screening results showed that the expression of RNF-11 and CTC-50DM5 gene in chordoma and normal tissue around the week were significantly different (P <0.001), of which RNF-11 gene expression in the tumor tissue Was significantly higher, while CTC-50DM5 gene expression was significantly increased in chordoma tissue. CONCLUSION: RNF-11 gene may be a tumor suppressor gene associated with chordoma, while CTC-50DM5 gene may be an oncogene associated with chordoma.