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目的 测定银杏叶多糖的组成、分子量、甙键类型及其对羟自由基的清除作用。方法 利用气相色谱法测定多糖组成；凝胶过滤法测定分子量；红外光谱法测定甙键类型。以·OH清除剂(甘露醇、苯甲酸)为对照，应用Fenton反应检测多糖对·OH的清除作用。结果 该多糖是由鼠李糖、阿拉伯糖、甘露糖、半乳糖组成，平均分子量为1..04×10６。红外光谱在835cm－１处有吸收。对·OH的清除作用达50%所需药物量以EC５０表示。多糖、甘露醇、苯甲酸的EC５０依次为867、3996和490μg/ml。结论 银杏叶多糖是以α甙键连接而成的杂多糖，该多糖对Fenton体系产生的·OH自由基有清除作用。
Objective To determine the composition, molecular weight, type of oxime bonds and their effects on the scavenging of hydroxyl radicals in Ginkgo biloba leaves. Methods The composition of polysaccharides was determined by gas chromatography, the molecular weight was determined by gel filtration method, and the type of oxime bond was determined by infrared spectroscopy. Using OH scavenger (mannitol, benzoic acid) as a control, Fenton reaction was used to detect the scavenging effect of polysaccharide on OH. Results The polysaccharide was composed of rhamnose, arabinose, mannose, galactose with an average molecular weight of 1..04×106. The infrared spectrum absorbs at 835 cm-1. The amount of drug required to eliminate 50% of OH is expressed as EC50. The EC50 for polysaccharides, mannitol, and benzoic acid was 867, 3996, and 490 μg/ml, respectively. Conclusion The polysaccharide of Ginkgo biloba L. is a heteropolysaccharide which is linked by α甙 linkage. This polysaccharide can scavenge OH radicals produced by Fenton system.