论文部分内容阅读
目的研究苦参、茯苓两种中药抗肝纤维化的作用及其机制。方法以生理盐水作为对照,将苦参和茯苓两种中药用于大鼠肝纤维化模型和大鼠肝星状细胞(HSC)。观察肝组织形态变化;检测血透明质酸酶、Ⅳ型胶原含量;MTT法检测HSC细胞增殖抑制率;免疫组化法检测基质金属蛋白酶-1(MMP-1)、基质金属蛋白酶组织抑制因子-1(TIMP-1)、转化生长因子(TGF)β1、血小板衍生生长因子(PDGF)的表达。结果与对照组比较,中药处理组的HSC细胞增殖抑制率升高(P<0.05);血清透明质酸、Ⅳ型胶原含量减少(P<0.05);肝组织TIMP-1、TGFβ1及PDGF的表达均减少(P<0.05)。结论两种中药均能减缓大鼠肝纤维化的发生,其机制可能与这些中药能下调TGFβ1及PDGF表达、抑制HSC增殖活化、促进细胞外基质降解、减少肝纤维结缔组织沉积有关。
Objective To study the anti-hepatic fibrosis effect and mechanism of sophora flavescens and Tuckahoe. Methods Saline was used as a control, and two traditional Chinese medicines, Sophora flavescens and Tuckahoe, were used in rat hepatic fibrosis model and rat hepatic stellate cells (HSC). The morphological changes of liver tissue were observed. The content of hyaluronidase and collagen Ⅳ were detected. The inhibition rate of HSC cell proliferation was detected by MTT assay. The expressions of MMP-1, MMP- 1 (TIMP-1), transforming growth factor (TGF) β1, and platelet-derived growth factor (PDGF). Results Compared with the control group, the inhibition rate of HSC cell proliferation was significantly increased (P <0.05), the levels of serum hyaluronic acid and type Ⅳ collagen were decreased (P <0.05), the expressions of TIMP-1, TGFβ1 and PDGF Decreased (P <0.05). Conclusion Both traditional Chinese medicines can slow down the occurrence of hepatic fibrosis in rats. The mechanism may be related to the fact that these Chinese herbs can down-regulate the expression of TGFβ1 and PDGF, inhibit the proliferation and activation of HSC, promote the degradation of extracellular matrix, and reduce the deposition of connective tissue in liver fibrosis.