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以苎麻(BoehmerianiveaL.)的试管苗为试材,由茎段诱导的愈伤组织分离原生质体,用MT+0.5mg·L-1KT+0.5mg·L-1GA3+0.02mg·L-1NAA+50mg·L-1水解乳蛋白+K8p+6mg·L-1蔗糖的基本培养基以低熔点琼脂糖(LMT)软包埋方式培养,5~6天后原生质体开始分裂,20~25天形成细胞团,随后产生愈伤组织,50~65天能再生成完整植株。对影响苎麻原生质体分离、培养及再生的主要因子进行比较研究的结果表明:用6%的纤维素酶分离苎麻的原生质体效果较好;蔗糖和葡萄糖是比较适宜的碳源;低熔点琼脂糖(LMT)软包埋培养方式,其原生质体分裂频率高、再生成株所需的时间短。
Protoplasts were isolated from stems of Boehmerianivea L. by using stem explants of Boehmerianivea L., and then hydrolyzed by MT + 0.5mg · L-1KT + 0.5mg · L-1GA3 + 0.02mg · L-1NAA + 50mg · L-1 The basic medium of milk protein + K8p + 6mg · L-1 sucrose was cultured by soft embedding with low melting point agarose (LMT). After 5-6 days, the protoplasts began to divide and formed cell masses 20-25 days, followed by callus formation. 50 ~ 65 days to regenerate into complete plants. The results showed that protoplast isolation of ramie with 6% cellulase was better; sucrose and glucose were more suitable carbon sources; low melting point agarose (LMT) soft-embedded culture mode, the protoplast division frequency is high, regeneration of the plant required time is short.