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用PCR法从大肠杆菌中调出长1.9kb的DNA片断,插入质粒pT77,核酸电泳和DNA测序分析结果证明该片段是GlyA目的基因。
The DNA fragment of 1.9 kb in length was amplified by PCR from E. coli and inserted into plasmid pT77. Nucleic acid electrophoresis and DNA sequencing analysis confirmed that the fragment was a GlyA gene.