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用ＰＣＲ法从大肠杆菌中调出长１．９ｋｂ的ＤＮＡ片断，插入质粒ｐＴ７７，核酸电泳和ＤＮＡ测序分析结果证明该片段是ＧｌｙＡ目的基因。
The DNA fragment of 1.9 kb in length was amplified by PCR from E. coli and inserted into plasmid pT77. Nucleic acid electrophoresis and DNA sequencing analysis confirmed that the fragment was a GlyA gene.