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目的:用指纹图谱对黄连解毒汤传统饮片汤剂和现代颗粒汤剂进行比较。方法:采用RP-HPLC梯度洗脱进行分析,色谱柱:Diamonsil(TM)-C18柱(250mm×4.6mm);流动相:水-甲醇-0.05%磷酸(梯度洗脱);检测波长:254nm;柱温:35℃。结果:所建立分析方法得到的HPLC色谱图有较好重现性。结论:不同厂家饮片汤剂、颗粒汤剂指纹图谱存在一定的差异。
Objective: To compare the traditional Chinese herbal decoction and modern granular decoction of Huanglianjiedutang with fingerprints. Methods: The gradient elution was performed by RP-HPLC. The column was Diamonsil(TM)-C18 column (250mm×4.6mm). The mobile phase was water-methanol-0.05% phosphoric acid (gradient elution). The detection wavelength was 254nm. Column temperature: 35°C. Results: HPLC chromatograms obtained by the established analytical method are reproducible. Conclusion: There are some differences in the fingerprints of the decoctions and decoctions of different manufacturers.