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目的:探讨NF-κB1和NF-κBIα基因多态性与乙肝表面抗原(HBsAg)阳性原发性肝细胞癌(hepatocellular carcino-ma,HCC)易患性之间的关系。方法:采用限制性片段长度多态性-聚合酶链反应(RFLP-PCR)方法检测乙肝表抗阳性HCC患者、慢性肝炎患者(CHB)、健康对照组的NF-κB1和NF-κBIα基因多态性。应用多因素Logistic回归综合分析年龄、性别、吸烟、饮酒及各位点基因型与肝癌易感性之间的关系。结果:HCC组中NF-κB1 ATTG2/ATTG2基因型频率明显高于健康对照组(OR=2.21,95%CI 1.25～3.88);以同时携带各位点野生型携带者为参照,健康对照组中同时携带NF-κB1 ATTG2/AT-TG2基因型和NF-κBIαGG基因型的个体患肝癌的危险性明显增高(OR=2.94,95%CI 1.03～8.44);携带NF-κB1 ATTG2/ATTG2基因型的肝炎患者患肝癌的危险性增高(OR=2.31,95%CI 1.22～4.38);多因素分析结果显示:男性患肝癌的危险性是女性的2.01倍(95%CI 1.19～3.41);吸烟者患肝癌的危险性是不吸烟者的1.79倍(95%CI 1.04～3.07);饮酒者患肝癌的危险性是不饮酒者的2.58倍(95%CI 1.50～4.43);NF-κB1 ATTG2/ATTG2基因型携带者患肝癌的危险性是野生型NF-κB1 ATTG1/ATTG1携带者的2.17倍(95%CI 1.23～3.85)。结论:NF-κB1 ATTG2/ATTG2基因型是原发性肝癌的危险因素,与NF-κBIα的GG基因型之间存在交互作用;性别、吸烟、饮酒也是肝癌的危险因素。
Objective: To investigate the relationship between NF-κB1 and NF-κBα gene polymorphisms and susceptibility to hepatitis B surface antigen (HBsAg) -positive hepatocellular carcinoma (HCC). Methods: RFLP-PCR was used to detect the polymorphisms of NF-κB1 and NF-κBα in hepatitis B virus-positive HCC patients, chronic hepatitis patients (CHB) and healthy controls Sex. Logistic regression analysis was used to analyze the relationship between age, sex, smoking, alcohol intake and locus genotypes and susceptibility to liver cancer. Results: The frequency of NF-κB1 ATTG2 / ATTG2 genotype in HCC group was significantly higher than that in healthy control group (OR = 2.21, 95% CI 1.25-3.88). At the same time, The risk of liver cancer was significantly higher in individuals with NF-κB1 ATTG2 / AT-TG2 genotypes and NF-κBααGG genotypes (OR = 2.94, 95% CI 1.03 ~ 8.44), hepatitis with NF-κB1 ATTG2 / ATTG2 genotype (OR = 2.31, 95% CI 1.22-4.38). Multivariate analysis showed that the risk of developing HCC in men was 2.01 times that of women (95% CI 1.19 to 3.41), and the incidence of HCC in smokers (95% CI 1.04 ~ 3.07). The risk of liver cancer in drinkers was 2.58 times higher than that in non-drinkers (95% CI 1.50-4.43). The NF-κB1 ATTG2 / ATTG2 genotype The risk of liver cancer in carriers was 2.17 times (95% CI 1.23 to 3.85) for carriers of wild-type NF-κB1 ATTG1 / ATTG1. CONCLUSION: NF-κB1 ATTG2 / ATTG2 genotype is a risk factor for primary hepatocellular carcinoma and interacts with GG genotype of NF-κBα. Gender, smoking and drinking are also risk factors for HCC.