论文部分内容阅读
目的:探讨二甲肼(DMH)诱发的大肠癌癌前病变模型大鼠的肠道内双歧杆菌数量与大肠癌癌前病变的关系。方法:应用双歧杆菌属特异性引物实时定量PCR,对实验第3与第9周8只模型组大鼠以及同年龄的7只正常对照组大鼠肠道中的双歧杆菌数量进行检测。结果:第9周的正常对照组大鼠未见明显异常隐窝灶(aberrant crypts foci,ACF),同年龄的模型组平均每只大鼠大肠可形成(37.7±2.6)个ACF。第3周正常对照组与同年龄的模型组大鼠肠道双歧杆菌数量差异无统计学意义,P=0.652;第9周模型组大鼠肠道双歧杆菌数量低于同年龄的正常对照组,但差异无统计学意义,P=0.090。结论:肠道内双歧杆菌的数量与大鼠大肠癌癌前病变没有明显相关性。
Objective: To investigate the relationship between the number of intestinal bifidobacteria and the precancerous lesions of colorectal cancer induced by dimethyl hydrazine (DMH) -induced colorectal cancer precancerous lesion in rats. Methods: Bifidobacterium specific primers were used to detect bifidobacteria in the intestine of 8 rats in model group and 7 normal control rats at the third and ninth week of the experiment. Results: No aberrant crypts foci (ACF) were observed in the normal control group at week 9, while the same model rats at the same age formed an average of (37.7 ± 2.6) ACFs per rat large intestine. The number of bifidobacteria in the model group at the third week was no significant difference between the normal control group and the model group at the same age (P = 0.652). The number of bifidobacteria in the model group at the 9th week was lower than that of the normal control Group, but the difference was not statistically significant, P = 0.090. Conclusion: There is no significant correlation between the amount of bifidobacteria in intestine and the precancerous lesions of colorectal cancer in rats.