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目的:探讨红景天苷(salidroside,Sal)对帕金森病(Parkinson’s disease,PD)小鼠黑质多巴胺转运蛋白(dopamine transporter,DAT)表达的影响。方法:将神经毒素1-甲基-4苯基-1,2,3,6-四氢吡啶(1-methyl-4-phen-yl-1,2,3,6-tetrahydropyridine,MPTP)注入C57BL/6小鼠腹腔内,制备PD模型,Rotarod实验和游泳实验观察小鼠行为学变化,免疫荧光组织化学法检测黑质DAT阳性神经元数目的变化。结果:行为学实验结果显示,Rotarod实验中模型组小鼠在滚轴上运动的时间明显短于正常组(P<0.01),给予不同剂量的Sal后,小鼠在滚轴上运动的时间有所延长,并呈剂量依赖性(P<0.05,P<0.01)。游泳实验结果与Rotarod结果一致,模型组小鼠游泳时间明显短于正常组(P<0.01),给予不同剂量Sal后,小鼠游泳时间不同程度增加(P<0.05,P<0.01)。免疫荧光结果显示,模型组小鼠黑质中DAT阳性神经元的数目明显少于正常组(P<0.01),而给予不同剂量Sal干预后,小鼠DAT阳性神经元数目的减少有所恢复(P<0.05,P<0.01)。结论:Sal可以改善PD小鼠行为协调能力,提高DAT阳性神经元存活的数目,并呈剂量依赖性,表明Sal对多巴胺(dopamine,DA)能神经元具有一定的保护作用。
Objective: To investigate the effect of salidroside (Sal) on dopamine transporter (DAT) expression in substantia nigra of Parkinson’s disease (PD) mice. Methods: The neurotoxin 1-methyl-4-phen-yl-1,2,3,6-tetrahydropyridine (MPTP) was injected into C57BL PD mice were prepared by intraperitoneal injection of PDMA / 6 mice. Rotarod test and swimming test were used to observe the behavioral changes in mice. Immunofluorescence staining was used to detect the number of substantia nigra DAT positive neurons. Results: The results of behavioral experiments showed that the mice in the model group of Rotarod exercise time on the roller was significantly shorter than that of the normal group (P <0.01). After the mice were given different doses of Sal, the mouse movement on the roller was Prolonged and in a dose-dependent manner (P <0.05, P <0.01). The result of swimming experiment was consistent with that of Rotarod. The swimming time of mice in the model group was significantly shorter than that of the normal group (P <0.01). After different doses of Sal, the swimming time of mice increased to different extents (P <0.05, P <0.01). Immunofluorescence results showed that the number of DAT positive neurons in substantia nigra of model group was significantly less than that of normal group (P <0.01), while the decrease of DAT positive neurons in mice was restored P <0.05, P <0.01). Conclusion: Sal can improve the behavior coordination ability of PD mice and increase the survival of DAT positive neurons in a dose-dependent manner, indicating that Sal can protect dopamine (DA) neurons.