冰冻连续切片、细胞和髓鞘间片分染法是研究动物脑结构的一种很好的方法。采取这种方法,可以用一只动物的脑制成细胞染色和髓鞘染色两种连续切片标本,既可以分别观察核群和纤维,又可对比两者的配布,有独到的优点。1979年,我们用上海产半导体致冷器冰冻大白鼠脑块,在普通手摇切片机上作连续切片,隔片分组,分别按Nissl法与Spielmeyer法浸染神经元胞体和髓鞘。经过多次实践,初步获得成功,制出了一脑双染、连续对照的切片。不过,当时切染的质量不够高,如切得厚薄不一致,染得浓淡不均 Frozen serial sections, cell and myelin interlaminar method are a good way to study the brain structure of animals. In this way, we can make two serial sections of cell stained cells and myelin stained with one animal’s brain, which can observe the nuclear group and the fiber separately and compare the two with the unique advantages. In 1979, we frozen the brains of rats with a Shanghai-made semiconductor cooler and sliced ​​them on a normal hand-cranked microtome. The septa were divided into groups, and the somatic cell bodies and myelin sheaths were disseminated according to the Nissl method and the Spielmeyer method respectively. After many practices, the initial success, made a brain double staining, continuous control of the slice. However, at that time, the quality of the cut was not high enough, such as inconsistencies in cut thickness and inconsistencies in shade