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目的:探讨加减木防己汤对缺血性心力衰竭(ischemic heart failure IHF)大鼠心室重构的影响及其作用机制。方法:60只雄性SD大鼠,冠脉结扎法制备IHF模型,选取6只为假手术组(只穿线不结扎),将造模成功大鼠随机分为模型组(6只)、西药组(6只)、中药高剂量组(6只)、中药中剂量组(6只)及中药低剂量组(6只)5组。假手术组和模型组给予1.0 m L/(100 g·d)纯净水灌胃;中药高、中、低剂量组(8、4、2g/m L)分别予等体积加减木防己汤灌胃;西药组予5 mg/(100 g·d)卡托普利灌胃,持续4周。心脏超声、血浆TNF-α、AngⅡ、IL-6、ET-1浓度测定及免疫组化测SERCA2a含量。结果:与假手术组比较,模型组的射血分数(EF)、实际面积和IOD阳性指数明显降低(P<0.05),左室收缩末期内径(LVS)、左室后壁厚度(LVPWD)明显升高(P<0.05);与模型组比较,西药对照组、中药低、中、药高剂量组EF明显升高(P<0.05),中药低、中、高剂量组LVS明显下降(P<0.05);中药3组室间隔厚度(IVSD)、LVPWD无明显改变(P>0.05),中药高剂量组实际面积明显升高(P<0.05),中药中剂量组IOD阳性指数明显升高(P<0.05);与西药对照组比较,中药高剂量组EF明显升高(P<0.05),中药中、高剂量组LVS明显下降(P<0.05),中药3个剂量组实际面积和IOD阳性指数无明显改变(P>0.05)。结论:西药和中药均能改善大鼠心功能,主要表现为对收缩功能改善;中高剂量中药在改善收缩功能方面优于西药;中高剂量中药能显著增加心肌SERCA2a含量,但中药未能改善心肌重构。
Objective: To investigate the effect and the mechanism of add and subtract mugandi decoction on ventricular remodeling in rats with ischemic heart failure (IHF). Sixty male Sprague-Dawley rats were randomly divided into model group (6 rats), western medicine group (6 rats) and western medicine group 6 high-dose group, 6 medium-dose traditional Chinese medicine group and 6 low-dose traditional Chinese medicine group. The sham-operated group and model group were given pure water of 1.0 m L / (100 g · d), respectively. The rats in high, medium and low dose groups (8, 4 and 2 g / m L) Stomach; Western medicine group was given captopril 5 mg / (100 g · d) for 4 weeks. The levels of serum TNF-α, AngⅡ, IL-6 and ET-1 were measured by echocardiography, and the content of SERCA2a was measured by immunohistochemistry. Results: Compared with the sham operation group, the ejection fraction (EF), actual area and IOD positive index of the model group were significantly decreased (P <0.05), LVS and LVPWD were significantly (P <0.05). Compared with the model group, the EF of the western medicine control group and the traditional Chinese medicine low, middle and high dose group were significantly increased (P <0.05), while the LVS of the low, middle and high dose Chinese medicine group were significantly decreased (P < 0.05). The IVSD and LVPWD did not change significantly in the three groups (P> 0.05), and the actual area of the Chinese medicine high-dose group was significantly increased (P <0.05) (P <0.05). Compared with the western medicine control group, the EF of high dose group of traditional Chinese medicine was significantly increased (P <0.05), LVS of middle and high dose of traditional Chinese medicine group was significantly decreased (P <0.05), and the actual area and IOD positive index No significant change (P> 0.05). Conclusion: Western medicine and traditional Chinese medicine can improve heart function of rats, mainly for the improvement of systolic function; middle and high dose of traditional Chinese medicine is better than Western medicine in improving systolic function; middle and high dose of traditional Chinese medicine can significantly increase the content of SERCA2a in myocardium, Structure.